Preliminary phytochemical screening, quantitative analysis, of antioxidant and antibacterial activity of crude plant extracts from Citrus Sinensis l.

Abstract

The present study of Consecutive describes ofn- Hexane, Petroleum ether, Ethyl acetate, Acetone, Methanol, plant leaf extract of the Citrus sinensis L. for investigated for the preliminary phytochemical analysis, the polar and non-polar solvent was screened for the presence of alkaloids, Flavonoids, saponins, glycosides, anthraquinone, phenol, tannins. Antioxidant activity DPPH radical is scavenged by antioxidants through the donation of a proton forming the reduced DPPH. ABTS+ radical scavenging activity ABTS+ (2, 2΄ Azinobis (3-ethyl benzo-thizoline-6-sulfonic acid. Antimicrobial activity different solvent in highest inhibition zone for methanol extract the microorganisms for (gram – positive) Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumonia, Bacillus megaterium, Corynebacteriumdiphtheria. (Gram-negative) Klebsiella pnemonia, Escherichia coli, pseudomonas aeruginosa, proteusvolgaris, pseudomonas fluorescens, pseudomonas veronii. Citrus sinensis L. Methanol were found be most effective showing the largest zone inhibition against. Further studies indicated that the gas chromatography compound analysis to the fragments from 40 to 600 Da. The spectrums of the components were compared with the database of spectrum of known components stored in the GC – MS.