Biosynthesis of ZNO nanoparticles (ZNO-NPS) from potential fungi and their antifungal activity against Helminthosporium oryzae

Abstract

In the present investigation stated that the nano science is a new interdisciplinary subject that depends on the fundamental properties of nano size objectives. The present study the biosynthesis of nano particles from some specific fungi like Aspergillus Niger and A. flavus, were performed. It was12.12±3.12 and 15.2±4.65 μg/ml found to be recognized with respective fungi. The effect of synergetic effect of antagonistic properties of potential fungi A. flavus and ZnO-NPs was approached and maximum antagonistic effect in 75μl:30μl was 14.72±6.02mm zone of inhibition recorded and minimum antagonistic effect of 25μl:10μl concentration of A. flavus secondary metabolites with ZnO-NPs was 08.07±0.09mm zone of the inhibition represented against Helminthosporium oryzae pathogen. The A.niger potential fungi with silver nano particles of synergistic effect were maximum at 75μl:30μl concentration in 12.10±4.18mm zone of the inhibition whereas minimum 25μl:10μl was 09.05±1.01mm zone of inhibition determined against Helminthosporium oryzae respectively. In the case of synergistic effect of potential fungi A. flavus with A. niger +ZnO-NPs at 75μl:30μl was 15.45±5.91mm zone of inhibition recognized and more effective antagonistic activity respectively whereas minimum antifungal activity of 50μl:20μl concentration was 10.03±2.08 from this work, the synergistic effect of A. flavus, A. niger and ZnO-NPs was excellent antagonistic properties against H. oryzae. Hence, the A. flavus and A. Niger with silver nano particles were excellent candidate for biocontrol agent against H. oryzae.