Green synthesis of copper nanoparticle from Cordia dichotoma leaf extract and its antimicrobial activity

Abstract

The development of nanotechnology is making the interest of researchers towards synthesis of nanoparticles for the bio application. Here copper nanoparticles were synthesized using Cordia dichotoma plant leaf extract. The leaf extract acts as both reducing and capping agent. The synthesized copper nanoparticles were confirmed by the change of colour after addition of leaf extract into the Copper Sulfate solution. The biosynthesized CuNPs were characterized by using UV-Vis analysis, Fourier Transform Infrared analysis (FTIR), X-ray diffraction analysis (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray analysis (EDX) and Transmission Electron Microscopy (TEM) analysis. The synthesized CuNPs were in cubical structure with the particle size in the range between 50-100 nm. The antibacterial test conducted on E. coli, Pseudomonas aeruginosa and Staphylococcus aureus, showed good zone of inhibitions 9.60, 9.45 and 9.50 mm, respectively, proving potentiality of g-Cu NPs as a remedy for infectious diseases caused by tested pathogens. Antibacterial and antifungal activities were performed by disk diffusion method. Cu NPs showed remarkable activity against bacterial strains.