Green synthesis and characterization of hydroxyapatite bio composite using Azadirachta indica A. Juss. bark extract and in-vitro biocompatibility with human osteoblasts cells

Abstract

Hydroxyapatite (HA) is good biocompatibility and bioactivity it’s excellent candidate for bone repair and substitution due to Ca/P ratio similar to natural bone. The the present study is synthesis HA by a wet chemical method using synthetic precursor composite with neem plant (Azadirachta indica) bark extract (AI) and to evaluate the antibacterial activity. Synthesised HA was characterised by using X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FT-IR) and Scanning electron microscope (SEM). The conformation of HA biocomposite was fabricated to polycrystalline hydroxyapatite exhibit hexagonal crystal structure. In addition to that, HA composite to analysis gram positive and gram negative bacteria such as Escherichia coli, Bacillus subtilis, and Staphylococcus aureus was evaluated by agar and disc diffusion method. Cytotoxicity activity was measured by using cell proliferation activity was observed on osteoblast MG-63 cell line. This study suggested that - synthesized biocomposite of HA with neem bark extract an effective substitute for synthetic starting material to bone tissue engineering.