Characterization of amylolytic bacteria Bacillus subtilis LC4 isolated from Sita kund hot spring, Bihar, India

Abstract

Microbial amylases share one quarter of industrial enzyme market. Their wide industrial application and ongoing demand has encouraged researchers to explore for newer and cheaper sources. The present study aims at isolation and characterization of amylase producing bacteria from water samples of Sita kund hot spring, Bihar, India. A total of 08 bacteria were isolated of which 04 were amylase positive. Among positive isolates, LC4 which showed maximum halo zone as well highest amylase production was selected for further study. Based on phenotypic and genotypic characterization, it was identified as Bacillus subtilis LC4 (Accession no: MH583045). Further effect of physical parameters on amylase production were optimized via one variable at a time approach under submerged fermentation condition. The strain showed maximum amylase production after 48 h of incubation, 7.0 pH and 45ºC. The results were encouraging and signifies hot spring as a sink of potent source of extracellular enzymes.